| Open flow cytometer with the combination of 3D hydrodynamic single cell focusing and confocal laser-induced fluorescence detection | |
Wu, Chengxin1; Wei, Xing1; Men, Xue1; Xu, Yulong1; Bai, Junjie1; Wang, Yu1; Zhou, L(周雷)2 ; Yu, Yong-Liang1; Xu, Zhang-Run1; Chen, Ming-Li1; Wang, Jian-Hua1 | |
| 2023-06-01 | |
| Source Publication | TALANTA Impact Factor & Quartile |
| ISSN | 0039-9140 |
| Volume | 258 |
| Abstract | Flow cytometry is among the most powerful tools for single-cell analysis, while the high cost and mechanical complexity of the commercial instrumentation limit the applications in personalized single-cell analysis. For this issue, we hereby construct an open and low-cost flow cytometer. It is highly compact to integrate the functions of (1) single cell aligning by a lab-made modularized 3D hydrodynamic focusing device, and (2) fluorescence detection of the single cells by a confocal laser-induced fluorescence (LIF) detector. The ceiling cost of the entire hardware for the LIF detection unit and 3D focusing device is $ 3200 and $ 400 respectively. A sheath flow velocity of 150 mu L/min produces a focused sample stream of 17.6 mu m x 14.6 mu m at sample flow of 2 mu L/min, based on the LIF response frequency and the laser beam spot diameter. The assay performance of the flow cy-tometer was evaluated by characterizing fluorescent microparticles and acridine orange (AO) stained HepG2 cells, producing throughputs of 40.5/s and 6.2/s respectively. Favorable assay precision and accuracy were demonstrated by the agreement of frequency histogram with imaging analysis, and good Gaussian-like distri-butions of fluorescent microparticles and AO-stained HepG2 cells. Practically, the flow cytometer was success-fully applied for the evaluation of ROS generation in single HepG2 cells. |
| Keyword | Single-cell analysis Sheath flow Flow cytometry Laser-induced fluorescence High-throughput |
| Publisher | ELSEVIER |
| DOI | 10.1016/j.talanta.2023.124424 |
| Indexed By | SCIE |
| Language | 英语 |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Analytical |
| WOS ID | WOS:000972566900001 |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.lzu.edu.cn/handle/262010/501572 |
| Collection | 化学化工学院 |
| Corresponding Author | Chen, Ming-Li; Wang, Jian-Hua |
| Affiliation | 1.Northeastern Univ, Coll Sci, Res Ctr Analyt Sci, Dept Chem, Shenyang 110819, Peoples R China; 2.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China |
| Recommended Citation GB/T 7714 | Wu, Chengxin,Wei, Xing,Men, Xue,et al. Open flow cytometer with the combination of 3D hydrodynamic single cell focusing and confocal laser-induced fluorescence detection[J]. TALANTA,2023,258. |
| APA | Wu, Chengxin.,Wei, Xing.,Men, Xue.,Xu, Yulong.,Bai, Junjie.,...&Wang, Jian-Hua.(2023).Open flow cytometer with the combination of 3D hydrodynamic single cell focusing and confocal laser-induced fluorescence detection.TALANTA,258. |
| MLA | Wu, Chengxin,et al."Open flow cytometer with the combination of 3D hydrodynamic single cell focusing and confocal laser-induced fluorescence detection".TALANTA 258(2023). |
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