兰州大学机构库 >基础医学院
Generation and comprehensive analysis of Synechococcus elongatus–Aspergillus nidulans co-culture system for polyketide production
Feng, J(冯杰)1; Li, Jingwei1; Liu, Dongxia1; Xin, Yuxian1; Sun, Jingrong1; Yin, Wen-Bing2; Li, TT(李婷婷)1
2023-12-01
Source PublicationBiotechnology for Biofuels and Bioproducts   Impact Factor & Quartile
Volume16Issue:1
page numbers12
AbstractBackground: Artificial microbial consortia composed of heterotrophic and photoautotrophic organisms represent a unique strategy for converting light energy and carbon dioxide into high-value bioproducts. Currently, the types of desired bioproducts are still limited, and microbial fitness benefit rendered by paired partner generally needs to be intensified. Exploring novel artificial microbial consortia at a laboratory scale is an essential step towards addressing this unmet need. This study aimed to conduct and analyze an artificial consortium composed of cyanobacterium Synechococcus elongatus FL130 with the filamentous fungus Aspergillus nidulans TWY1.1 for producing fungi-derived secondary metabolite of polyketide neosartoricin B. Results: Polyketide-producing A. nidulans TWY1.1 substantially ameliorated the growth and the survival of sucrose-secreting cyanobacterium S. elongatus FL130 in salt-stressed environments. Besides sucrose, comparable amounts of other carbohydrates were released from axenically cultured FL130 cells, which could be efficiently consumed by TWY1.1. Relative to axenically cultured FL130, less glycogen was accumulated in FL130 cells co-cultured with TWY1.1, and the glycogen phosphorylase gene catalyzing the first step for glycogen degradation had two-fold expression. Different from axenically cultured filamentous fungi, abundant vacuoles were observed in fungal hyphae of TWY1.1 co-cultured with cyanobacterium FL130. Meanwhile, FL130 cells displayed a characteristic pattern of interacting with its heterotrophic partner, densely dispersing along certain hyphae of TWY1.1. Finally, polyketide neosartoricin B was produced from TWY1.1 in FL130-TWY1.1 co-cultures, which was tightly adjusted by nitrogen level. Conclusion: Overall, the results thoroughly proved the concept of pairing cyanobacteria with filamentous fungi to build artificial consortia for producing fungi-derived biomolecules. © 2023, The Author(s).
KeywordCarbon dioxide Ketones Metabolites Phosphorylation Sugar (sucrose) Aspergillus nidulans Bioproducts Co-cultures Cyanobacterium Filamentous fungus Glycogen Microbial consortium Polyketides Secondary metabolites Synechococcus elongatus
PublisherBioMed Central Ltd
DOI10.1186/s13068-023-02283-6
Indexed ByEI ; SCIE
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectBiotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000941953400001
EI Accession Number20231013670831
EI KeywordsAspergillus
EI Classification Number461.9 Biology ; 802.2 Chemical Reactions ; 804.1 Organic Compounds ; 804.2 Inorganic Compounds ; 822.3 Food Products
Original Document TypeJournal article (JA)
PMID 36859469
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/529762
Collection基础医学院
Corresponding AuthorLi, Tingting
Affiliation
1.School of Basic Medical Sciences, Lanzhou University, Lanzhou; 730000, China;
2.State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing; 100101, China
First Author AffilicationSchool of Basic Medical Sciences
Corresponding Author AffilicationSchool of Basic Medical Sciences
Recommended Citation
GB/T 7714
Feng, Jie,Li, Jingwei,Liu, Dongxia,et al. Generation and comprehensive analysis of Synechococcus elongatus–Aspergillus nidulans co-culture system for polyketide production[J]. Biotechnology for Biofuels and Bioproducts,2023,16(1).
APA Feng, Jie.,Li, Jingwei.,Liu, Dongxia.,Xin, Yuxian.,Sun, Jingrong.,...&Li, Tingting.(2023).Generation and comprehensive analysis of Synechococcus elongatus–Aspergillus nidulans co-culture system for polyketide production.Biotechnology for Biofuels and Bioproducts,16(1).
MLA Feng, Jie,et al."Generation and comprehensive analysis of Synechococcus elongatus–Aspergillus nidulans co-culture system for polyketide production".Biotechnology for Biofuels and Bioproducts 16.1(2023).
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