兰州大学机构库
Comprehensive Analysis of Microbiome, Metabolome, and Transcriptome Revealed the Mechanisms of Intestinal Injury in Rainbow Trout under Heat Stress
Zhou, Changqing1,2; Gao, Pan1; Wang, JL(王建林)1
2023-05-10
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   Impact Factor & Quartile
ISSN1661-6596
Volume24Issue:10
page numbers26
AbstractGlobal warming is one of the most common environmental challenges faced by cold-water fish farming. Intestinal barrier function, gut microbiota, and gut microbial metabolites are significantly altered under heat stress, posing serious obstacles to the healthy artificial culture of rainbow trout. However, the molecular mechanisms underlying intestinal injury in rainbow trout under heat stress remain unclear. In the present study, the optimal growth temperature for rainbow trout (16 degrees C) was used for the control group, and the maximum temperature tolerated by rainbow trout (24 degrees C) was used for the heat stress group, which was subjected to heat stress for 21 days. The mechanism of intestinal injury in rainbow trout under heat stress was explored by combining animal histology, 16S rRNA gene amplicon sequencing, ultra-high performance liquid chromatography-mass spectrometry, and transcriptome sequencing. The results showed that the antioxidant capacity of rainbow trout was enhanced under heat stress, the levels of stress-related hormones were significantly increased, and the relative expression of genes related to heat stress proteins was significantly increased, indicating that the heat stress model of rainbow trout was successfully established. Secondly, the intestinal tract of rainbow trout showed inflammatory pathological characteristics under heat stress, with increased permeability, activation of the inflammatory factor signaling pathway, and increased relative expression of inflammatory factor genes, suggesting that the intestinal barrier function was impaired. Thirdly, heat stress caused an imbalance of intestinal commensal microbiota and changes in intestinal metabolites in rainbow trout, which participated in the stress response mainly by affecting lipid metabolism and amino acid metabolism. Finally, heat stress promoted intestinal injury in rainbow trout by activating the peroxisome proliferator-activated receptor- ff signaling pathway. These results not only expand the understanding of fish stress physiology and regulation mechanisms, but also provide a scientific basis for healthy artificial culture and the reduction of rainbow trout production costs.
Keywordrainbow trout heat stress gut microbiota barrier function multi-omics
PublisherMDPI
DOI10.3390/ijms24108569
Indexed BySCIE
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000997372200001
Original Document TypeArticle
PMID 37239914
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/530165
Collection兰州大学
草地农业科技学院
Corresponding AuthorWang, Jianlin
Affiliation
1.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Grassland Agr Engn Ctr, State Key Lab Grassland Agroecosystems,Minist Agr, Lanzhou 730020, Peoples R China;
2.Lanzhou Univ, Coll Ecol, Lanzhou 730000, Peoples R China
First Author AffilicationCollege of Pastoral Agriculture Science and Technology;  Lanzhou University
Corresponding Author AffilicationCollege of Pastoral Agriculture Science and Technology
Recommended Citation
GB/T 7714
Zhou, Changqing,Gao, Pan,Wang, Jianlin. Comprehensive Analysis of Microbiome, Metabolome, and Transcriptome Revealed the Mechanisms of Intestinal Injury in Rainbow Trout under Heat Stress[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2023,24(10).
APA Zhou, Changqing,Gao, Pan,&Wang, Jianlin.(2023).Comprehensive Analysis of Microbiome, Metabolome, and Transcriptome Revealed the Mechanisms of Intestinal Injury in Rainbow Trout under Heat Stress.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,24(10).
MLA Zhou, Changqing,et al."Comprehensive Analysis of Microbiome, Metabolome, and Transcriptome Revealed the Mechanisms of Intestinal Injury in Rainbow Trout under Heat Stress".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24.10(2023).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[Zhou, Changqing]'s Articles
[Gao, Pan]'s Articles
[Wang, Jianlin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhou, Changqing]'s Articles
[Gao, Pan]'s Articles
[Wang, Jianlin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhou, Changqing]'s Articles
[Gao, Pan]'s Articles
[Wang, Jianlin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.