| Transcriptomic and metabolomic perspectives for the growth of alfalfa (Medicago sativa L.) seedlings with the effect of vanadium exposure | |
| Wu, Zhen-zhong1,2; Gan, Zhi-wei1; Zhang, You-xian2; Chen, Si-bei1; Gan, Chun-dan1; Yang, Kai1; Yang, Jin-yan1 | |
| 2023-09 | |
| Source Publication | CHEMOSPHERE Impact Factor & Quartile |
| ISSN | 0045-6535 |
| Volume | 336 |
| Abstract | Hitherto, the effect of vanadium on higher plant growth remains an open topic. Therefore, nontargeted metabolomic and RNA-Seq profiling were implemented to unravel the possible alteration in alfalfa seedlings subjected to 0.1 mg L−1 (B group) and 0.5 mg L−1 (C group) pentavalent vanadium [(V(V)] versus control (A group) in this study. Results revealed that vanadium exposure significantly altered some pivotal transcripts and metabolites. The number of differentially expressed genes (DEGs) markedly up- and down-regulated was 21 and 23 in B_vs_A, 27 and 33 in C_vs_A, and 24 and 43 in C_vs_B, respectively. The number for significantly up- and down-regulated differential metabolites was 17 and 15 in B_vs_A, 43 and 20 in C_vs_A, and 24 and 16 in C_vs_B, respectively. Metabolomics and transcriptomics co-analysis characterized three significantly enriched metabolic pathways in C_vs_A comparing group, viz., α-linolenic acid metabolism, flavonoid biosynthesis, and phenylpropanoid biosynthesis, from which some differentially expressed genes and differential metabolites participated. The metabolite of traumatic acid in α-linolenic acid metabolism and apigenin in flavonoid biosynthesis were markedly upregulated, while phenylalanine in phenylpropanoid biosynthesis was remarkably downregulated. The genes of allene oxide cyclase (AOC) and acetyl-CoA acyltransferase (fadA) in α-linolenic acid metabolism, and chalcone synthase (CHS), flavonoid 3′-monooxygenase (CYP75B1), and flavonol synthase (FLS) in flavonoid biosynthesis, and caffeoyl-CoA O-methyltransferase (CCoAOMT) in phenylpropanoid biosynthesis were significantly downregulated. While shikimate O-hydroxycinnamoyltransferase (HCT) in flavanoid and phenylpropanoid biosynthesis were conspicuously upregulated. Briefly, vanadium exposure induces a readjustment yielding in metabolite and the correlative synthetic precursors (transcripts/unigenes) in some branched metabolic pathways. This study provides a practical and in-depth perspective from transcriptomics and metabolomics in investigating the effects conferred by vanadium on plant growth and development. © 2023 Elsevier Ltd |
| Keyword | Amino acids Biochemistry Biomolecules Flavonoids Genes Metabolism Metabolites Plants (botany) RNA Seed Co-analysis Flavonoid Linolenic acids Metabolomes Metabolomics Phenylpropanoids Plant Plant growth Transcriptomes Transcriptomics |
| Publisher | Elsevier Ltd |
| DOI | 10.1016/j.chemosphere.2023.139222 |
| Indexed By | EI |
| Language | 英语 |
| EI Accession Number | 20232614298282 |
| EI Keywords | Biosynthesis |
| EI Classification Number | 461.2 Biological Materials and Tissue Engineering ; 461.8 Biotechnology ; 461.9 Biology ; 801.2 Biochemistry ; 802.2 Chemical Reactions ; 804.1 Organic Compounds ; 821.4 Agricultural Products |
| Original Document Type | Journal article (JA) |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.lzu.edu.cn/handle/262010/532086 |
| Collection | 兰州大学 |
| Corresponding Author | Yang, Jin-yan |
| Affiliation | 1.College of Architecture and Environment, Sichuan University, Sichuan, Chengdu; 610065, China; 2.College of Earth and Environmental Sciences, Lanzhou University, Gansu, Lanzhou; 730000, China |
| First Author Affilication | Lanzhou University |
| Recommended Citation GB/T 7714 | Wu, Zhen-zhong,Gan, Zhi-wei,Zhang, You-xian,et al. Transcriptomic and metabolomic perspectives for the growth of alfalfa (Medicago sativa L.) seedlings with the effect of vanadium exposure[J]. CHEMOSPHERE,2023,336. |
| APA | Wu, Zhen-zhong.,Gan, Zhi-wei.,Zhang, You-xian.,Chen, Si-bei.,Gan, Chun-dan.,...&Yang, Jin-yan.(2023).Transcriptomic and metabolomic perspectives for the growth of alfalfa (Medicago sativa L.) seedlings with the effect of vanadium exposure.CHEMOSPHERE,336. |
| MLA | Wu, Zhen-zhong,et al."Transcriptomic and metabolomic perspectives for the growth of alfalfa (Medicago sativa L.) seedlings with the effect of vanadium exposure".CHEMOSPHERE 336(2023). |
| Files in This Item: | There are no files associated with this item. | ||||
