兰州大学机构库
A promising tool for clinical diagnostics: Dual-emissive carbonized polymer dots based cross-linking enhanced emission for sensitive detection of alkaline phosphatase and butyrylcholinesterase
Li, Tingting1,2; Wang, Dan1,2; Hu, Jing1,2; Fu, Xu3; Ji, Yibing1,2; Li, Ruijun1,2
2023-10-15
Source PublicationBIOSENSORS & BIOELECTRONICS   Impact Factor & Quartile
ISSN0956-5663
Volume238
AbstractCompared with single signal readout, dual-signal readout commendably corrects the impact of systematic or background error, achieving more accurate results for the diagnosis of many diseases. This work aimed to design and prepare dual-emissive fluorescent probes for the construction of ratiometric fluorescence biosensors to detect liver disease biomarkers. Sodium alginate (SA) with numerous potential sub-fluorophores and active sites and 4,4′,4′′,4′′′-(porphine-5,10,15,20-tetrayl) tetrakis (benzoic acid) (TCPP) with macrocyclic conjugated structures were introduced to prepare the carbonized polymer dots (CPDs) with red/blue dual emission based on the cross-linking enhanced emission (CEE) effect and the luminescence of macrocyclic conjugated structures. The ratiometric fluorescence sensing systems were constructed by integrating the specific response of CPDs to Cu2+ and the affinity difference of Cu2+ to substrates or products of enzymes. The sensing systems, CPDs/Cu2+/PPi and CPDs/Cu2+/BTCh, were designed to detect liver disease biomarkers, alkaline phosphatase (ALP) and butyrylcholinesterase (BChE), respectively. The limit of detection for ALP and BChE was 0.35 U/L and 0.19 U/L, respectively. The proposed sensors were successfully applied to human serum samples from different health stages with satisfactory recoveries. These results demonstrate the successful design of a novel dual-emissive fluorescent probe and provide a feasible strategy for clinical detection. © 2023 Elsevier B.V.
KeywordBiomarkers Conjugated polymers Crosslinking Diagnosis Fluorophores Probes Sodium alginate ALkaline phosphatase Butyrylcholinesterase Carbonized polymer dot Cross linking Cross-linking enhanced emission Dual-emissive Enhanced Emission Fluorescent probes Polymer dots Signal readouts
PublisherElsevier Ltd
DOI10.1016/j.bios.2023.115576
Indexed ByEI
Language英语
EI Accession Number20233214496935
EI KeywordsBenzoic acid
EI Classification Number461.6 Medicine and Pharmacology ; 741.1 Light/Optics ; 801.4 Physical Chemistry ; 802.2 Chemical Reactions ; 804.1 Organic Compounds ; 815.1.1 Organic Polymers
Original Document TypeJournal article (JA)
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/532120
Collection兰州大学
第二临床医学院
Corresponding AuthorFu, Xu; Ji, Yibing; Li, Ruijun
Affiliation
1.Department of Analytical Chemistry, China Pharmaceutical University, Nanjing; 210009, China;
2.Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing; 210009, China;
3.Laboratory of Emergency Medicine, Lanzhou University Second Hospital, Gansu, Lanzhou; 730000, China
Corresponding Author AffilicationSecond Clinical School
Recommended Citation
GB/T 7714
Li, Tingting,Wang, Dan,Hu, Jing,et al. A promising tool for clinical diagnostics: Dual-emissive carbonized polymer dots based cross-linking enhanced emission for sensitive detection of alkaline phosphatase and butyrylcholinesterase[J]. BIOSENSORS & BIOELECTRONICS,2023,238.
APA Li, Tingting,Wang, Dan,Hu, Jing,Fu, Xu,Ji, Yibing,&Li, Ruijun.(2023).A promising tool for clinical diagnostics: Dual-emissive carbonized polymer dots based cross-linking enhanced emission for sensitive detection of alkaline phosphatase and butyrylcholinesterase.BIOSENSORS & BIOELECTRONICS,238.
MLA Li, Tingting,et al."A promising tool for clinical diagnostics: Dual-emissive carbonized polymer dots based cross-linking enhanced emission for sensitive detection of alkaline phosphatase and butyrylcholinesterase".BIOSENSORS & BIOELECTRONICS 238(2023).
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