兰州大学机构库
HMGB1对大鼠糖尿病足溃疡炎症的影响及机制分析
谢瑞敏1,2; 李想1,2; 李强强1,2; 魏建仝3; 王勇平1
2020-07-15
Source Publication现代生物医学进展   Impact Factor & Quartile
ISSN1673-6273
Volume20Issue:13Pages:2430-2434+2429
page numbers6
Abstract目的:分析高迁移率族蛋白1(High mobility group protein 1,HMGB1)对大鼠糖尿病足溃疡炎症的影响及机制。方法:经腹腔注射链脲佐菌素建立糖尿病大鼠模型,并在大鼠的双后足背部做一3 mm×7 mm的全层矩形皮肤缺损,接种质控菌株。将建模成功的大鼠随机分成:模型组(等量生理盐水)、低剂量组(12.5μg·kg-1·d-1)、中剂量组(25μg·kg-1·d-1)和高剂量组(50μg·kg-1·d-1),每组6只,每天单次腹腔注射,连续给药14 d。另取6只大鼠作为空白组(等量生理盐水)。于规定时间对各组大鼠创面愈合率、微血管密度(Microvascular density,MVD)、组织中血管内皮生长因子(Vascular endothelial growth factor,VEGF)、CD45、Toll样受体4(Toll like receptor 4,TLR4)、核因子κB (Nuclear factor kappa-B,NF-κB)、白介素-1β(Interleukin-1β,IL-1β)、白介素-6 (Interleukin-6,IL-6)、IL-8(Interleukin-8,IL-8)和肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)m RNA或蛋白表达量进行检测。结果:与对照组相比,模型组大鼠创面治愈率和微血管密度均较低,VEGF、CD45、TLR4、NF-κB、IL-1β、IL-6、IL-8和TNF-αm RNA或蛋白表达量均较高(P<0.05)。与模型组相比,三个剂量组大鼠创面治愈率和微血管密度均明显降低(P<0.05),VEGF、CD45、TLR4、NF-κB、IL-1β、IL-6、IL-8和TNF-αm RNA或蛋白表达量均较高(P<0.05)。且随着HMGB1注射剂量增大,创面愈合率、血管生成明显减少(P <0.05),炎症因子表达量显著升高(P <0.05)。结论:HMGB1对大鼠糖尿病足溃疡炎症有促进作用,机制可能与HMGB1/TLR4/NF-κB信号通路相关。
Keyword高迁移率族蛋白B1 糖尿病足 炎症 微血管密度
DOI10.13241/j.cnki.pmb.2020.13.006
URL查看原文
Language中文
Subtype学术期刊
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/556072
Collection兰州大学
第一临床医学院
Corresponding Author王勇平
Affiliation
1.兰州大学第一医院骨科;
2.兰州大学第一临床医学院;
3.河西学院附属张掖人民医院骨科
First Author AffilicationFirst Clinical School
Corresponding Author AffilicationFirst Clinical School
Recommended Citation
GB/T 7714
谢瑞敏,李想,李强强,等. HMGB1对大鼠糖尿病足溃疡炎症的影响及机制分析[J]. 现代生物医学进展,2020,20(13):2430-2434+2429.
APA 谢瑞敏,李想,李强强,魏建仝,&王勇平.(2020).HMGB1对大鼠糖尿病足溃疡炎症的影响及机制分析.现代生物医学进展,20(13),2430-2434+2429.
MLA 谢瑞敏,et al."HMGB1对大鼠糖尿病足溃疡炎症的影响及机制分析".现代生物医学进展 20.13(2020):2430-2434+2429.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[谢瑞敏]'s Articles
[李想]'s Articles
[李强强]'s Articles
Baidu academic
Similar articles in Baidu academic
[谢瑞敏]'s Articles
[李想]'s Articles
[李强强]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[谢瑞敏]'s Articles
[李想]'s Articles
[李强强]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.