兰州大学机构库
High-Throughput Drug Screening on Borrelia garinii and Borrelia afzelii Identified Hypocrellin A as an Active Drug Candidate Against Borrelia Species
Li, Tingting1; Xin, Yuxian1; Liu, Dongxia1; Sun, Jingrong1; Li, Jingwei1; Zhang, Ying2,3,6; Feng, Jie1,4,5
2023-06
Source PublicationINFECTIOUS MICROBES & DISEASES   Impact Factor & Quartile
ISSN2641-5917
Volume5Issue:2Pages:83-92
page numbers10
AbstractLyme disease (LD) is a tick-transmitted infection caused by Borrelia burgdorferi sensu lato species, which include B. burgdorferi, Borrelia afzelii and Borrelia garinii. The majority of patients with early LD can be cured by the standard treatment, yet some still suffer from posttreatment LD syndrome. The presence of Borrelia persisters has been proposed as a contributing factor, because they cannot be completely eradicated by the currently used antibiotics for LD. Finding new pharmaceuticals targeting Borrelia persisters is crucial for developing more effective treatments. Here, we first confirmed the existence of persisters in B. garinii and B. afzelii cultures and then conducted a high-throughput screening of a custom drug library against persister-rich stationary-phase B. garinii and B. afzelii cultures. Among 2427 compounds screened, hypocrellin A (HA), anthracycline class of drugs and topical antibiotics along with some other natural compounds were identified to have strong potential for killing persisters of B. garinii and B. afzelii. HA was the most active anti-Borrelia compound, capable of eradicating stationary-phase Borrelia persisters, in particular when combined with doxycycline and/or ceftriaxone. Liposoluble antioxidant vitamin E was found to antagonize the activity of HA, indicating HA's target is the cell membrane where HA triggers the generation of reactive oxygen species in the presence of light. HA was found to have distinct bactericidal activity against Borrelia species but had poor or no activity against gram-positive and gram-negative bacteria. Identification of the abovementioned drug candidates may help develop more effective therapies for LD.
Keyworddrug screening Borrelia afzelii Borrelia garinii persister hypocrellin A biofilm
PublisherLIPPINCOTT WILLIAMS & WILKINS
DOI10.1097/IM9.0000000000000115
Indexed ByESCI
Language英语
WOS Research AreaInfectious Diseases ; Microbiology
WOS SubjectInfectious Diseases ; Microbiology
WOS IDWOS:001020249900006
Original Document TypeArticle
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/568657
Collection兰州大学
Corresponding AuthorZhang, Ying; Feng, Jie
Affiliation
1.Lanzhou Univ, Sch Basic Med Sci, Lanzhou, Peoples R China;
2.Zhejiang Univ, Affiliated Hosp 1, State Key Lab Diag & Treatment Infect Dis, Sch Med, Hangzhou, Peoples R China;
3.Jinan Microecol Biomed Shandong Lab, Jinan, Peoples R China;
4.Lanzhou Univ, Coll Vet Med, State Key Lab Vet Etiol Biol, Lanzhou, Peoples R China;
5.Lanzhou Univ, Sch Basic Med Sci, 199 Donggang West Rd, Lanzhou 730000, Peoples R China;
6.Zhejiang Univ, Affiliated Hosp 1, State Key Lab Diag & Treatment Infect Dis, Sch Med, 79 Qingchun Rd, Hangzhou 310003, Peoples R China
Recommended Citation
GB/T 7714
Li, Tingting,Xin, Yuxian,Liu, Dongxia,et al. High-Throughput Drug Screening on Borrelia garinii and Borrelia afzelii Identified Hypocrellin A as an Active Drug Candidate Against Borrelia Species[J]. INFECTIOUS MICROBES & DISEASES,2023,5(2):83-92.
APA Li, Tingting.,Xin, Yuxian.,Liu, Dongxia.,Sun, Jingrong.,Li, Jingwei.,...&Feng, Jie.(2023).High-Throughput Drug Screening on Borrelia garinii and Borrelia afzelii Identified Hypocrellin A as an Active Drug Candidate Against Borrelia Species.INFECTIOUS MICROBES & DISEASES,5(2),83-92.
MLA Li, Tingting,et al."High-Throughput Drug Screening on Borrelia garinii and Borrelia afzelii Identified Hypocrellin A as an Active Drug Candidate Against Borrelia Species".INFECTIOUS MICROBES & DISEASES 5.2(2023):83-92.
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