兰州大学机构库
Shenqi formula delayed Alzheimer's disease-like symptoms by skn-1 pathway in Caernorhabditis elegans
Zhi, Dejuan1,2; Xu, Shuaishuai1; Zhang, Ling1; Li, Yuxuan1; Zhu, Hai1; Zhao, Chengmu1; Wang, Dongsheng1,2,3
2023-11-15
Online publication date2023-06
Source PublicationJOURNAL OF ETHNOPHARMACOLOGY   Impact Factor & Quartile
ISSN0378-8741 ; 1872-7573
Volume316
page numbers12
AbstractEthnopharmacological relevance: Shenqi formula is composed of Codonopsis pilosula (Cp) and Lycium barbarum (Lb), and it is traditionally used for promoting qi and nourishing the spleen, liver and kidney. Cp and Lb have been reported to improve cognitive performance in APP/PS1 mice, prevent the accumulation of A beta, and reduce the neurotoxicity of A beta to achieve the anti-Alzheimer's disease (AD) effect. Aim of the study: Shenqi formula was explored the therapeutic effect on Caenorhabditis elegans AD pathological model and the underlying mechanism of action. Materials and methods: Paralysis assay and serotonin sensitivity assay was used to detect whether Shenqi formula can alleviate AD paralysis phenotype, and then DPPH, ABTS, NBT and Fenton methods were applied to investigate the scavenging capacity to free radical, ROS, center dot O-2(-) and center dot OH of Shenqi formula in vitro. H2DCF-DA and MitoSOX (TM) Red were employed to measure ROS and center dot O-2(-) accumulation, respectively. RNAi was used to knock down the expression of skn-1 and daf-16 related to oxidative stress resistance signalling pathway. Fluorescence microscopy was used to record the expression of SOD-3::GFP, GST-4::GFP, SOD-1::YFP, and the nuclear translocation of SKN-1 and DAF-16. Western blot assay was carried out to test A beta monomers and oligomers. Results: Shenqi formula delayed the AD-like pathological characteristics in C. elegans, and the complete Shenqi formula was more effective than Cp or Lb alone. The effect of Shenqi formula on delaying worm paralysis was partially eliminated by skn-1 RNAi, but not daf-16 RNAi. Shenqi formula significantly inhibited the abnormal deposition of A beta protein, decreased A beta protein monomers and oligomers. It increased the expressions of gst-4, sod-1, and sod-3 similar to paraquat, companied by rise then fall of ROS and.O2 in AD worms. Conclusions: Shenqi formula at least partially depended on SKN-1 signalling pathway to exert its anti-AD effect, and it is potential to be used as a kind of health food to prevent the progress of AD.
KeywordShenqi formula AD C. elegans SKN-1 Anti-oxidative stress Pro-oxidant agent
PublisherELSEVIER IRELAND LTD
DOI10.1016/j.jep.2023.116741
Indexed BySCIE
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:001020408000001
Original Document TypeArticle
PMID 37290734
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/569164
Collection兰州大学
Corresponding AuthorWang, Dongsheng
Affiliation
1.Lanzhou Univ, Sch Pharm, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China;
2.Frontiers Sci Ctr Rare Isotopes, 222 Tianshui South Rd, Lanzhou 730000, Gansu, Peoples R China;
3.Lanzhou Univ, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China
Recommended Citation
GB/T 7714
Zhi, Dejuan,Xu, Shuaishuai,Zhang, Ling,et al. Shenqi formula delayed Alzheimer's disease-like symptoms by skn-1 pathway in Caernorhabditis elegans[J]. JOURNAL OF ETHNOPHARMACOLOGY,2023,316.
APA Zhi, Dejuan.,Xu, Shuaishuai.,Zhang, Ling.,Li, Yuxuan.,Zhu, Hai.,...&Wang, Dongsheng.(2023).Shenqi formula delayed Alzheimer's disease-like symptoms by skn-1 pathway in Caernorhabditis elegans.JOURNAL OF ETHNOPHARMACOLOGY,316.
MLA Zhi, Dejuan,et al."Shenqi formula delayed Alzheimer's disease-like symptoms by skn-1 pathway in Caernorhabditis elegans".JOURNAL OF ETHNOPHARMACOLOGY 316(2023).
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