兰州大学机构库
The enhancement in toxic potency of oxidized functionalized polyethylene-microplastics in mice gut and Caco-2 cells
Wang, Ji1; Tian, Huanbing1; Shi, Yongpeng1; Yang, Ying1; Yu, Feifei1; Cao, Hanwen1; Gao, Lan1; Liu, Mingxin2
2023-12-10
Online publication date2023-08
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT   Impact Factor & Quartile
ISSN0048-9697 ; 1879-1026
Volume903
page numbers12
AbstractMicroplastics (MPs) are inevitably oxidized in the environment, however, to date, no studies have discussed the biological toxicity of oxidized polyethylene (Ox-PE) MPs. In this study, oxidized low-density polyethylene (Ox-LDPE), a representative Ox-PE, was prepared using a selective oxidation method. The difference in toxicity between LDPE-MPs and Ox-LDPE-MPs were evaluated in C57BL/6 mice and Caco-2 cells. The proton nuclear magnetic resonance (1H NMR) and Fourier transform infrared (FTIR) spectroscopy analyses revealed that some hydrocarbon-containing groups were transformed into carboxyl and ketone groups during selective oxidation. In vivo experiment results showed that LDPE-MPs and Ox-LDPE-MPs exists in the intestinal (duodenum and colon) of mice, and Ox-LDPE-MPs caused more severe intestinal histological changes, oxidative stress, and inflammatory response. The gut microbiota data showed that the relative abundance of Lactobacillus decreased significantly in the LDPE-MP-and Ox-LDPE-MP-exposed groups (P < 0.05). The predicted Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway suggested that exposure to LDPE-MPs or Ox-LDPE-MPs inhibited glycan biosynthesis and metabolism in the flora (P < 0.05). In vitro experiment results showed that selective oxidation to LDPE promoted its uptake by cells and aggravated adverse effects on cells, including reduced cell viability, damaged cell membrane, oxidative stress, and mitochondrial depolarization. The major mechanism of the increased toxicity of Ox-LDPE-MPs may be its easier accumulation and the ionic effect of oxygen-containing functional groups. Overall, these findings provide insights on the differences in toxicity between LDPE-MPs and Ox-LDPE-MPs. They also provide new perspectives for understanding the biohazards of MPs, which are necessary to accurately assess the potential environmental and health risks of these plastic pollutants.
KeywordLow -density polyethylene microplastics Selective oxidation Toxicity evaluation Mice Caco-2 cells
PublisherELSEVIER
DOI10.1016/j.scitotenv.2023.166057
Indexed BySCIE
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:001066507100001
Original Document TypeArticle
PMID 37553056
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/569480
Collection兰州大学
Corresponding AuthorGao, Lan; Liu, Mingxin
Affiliation
1.Lanzhou Univ, Sch Life Sci, 222 South Tianshui Rd, Lanzhou 730000, Gansu, Peoples R China;
2.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, 222 South Tianshui Rd, Lanzhou 730000, Gansu, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ji,Tian, Huanbing,Shi, Yongpeng,et al. The enhancement in toxic potency of oxidized functionalized polyethylene-microplastics in mice gut and Caco-2 cells[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2023,903.
APA Wang, Ji.,Tian, Huanbing.,Shi, Yongpeng.,Yang, Ying.,Yu, Feifei.,...&Liu, Mingxin.(2023).The enhancement in toxic potency of oxidized functionalized polyethylene-microplastics in mice gut and Caco-2 cells.SCIENCE OF THE TOTAL ENVIRONMENT,903.
MLA Wang, Ji,et al."The enhancement in toxic potency of oxidized functionalized polyethylene-microplastics in mice gut and Caco-2 cells".SCIENCE OF THE TOTAL ENVIRONMENT 903(2023).
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