兰州大学机构库
Sodium arsenite exposure enhances H3K14 acetylation and impairs male spermatogenesis in rat testes
Li, Xiangli1; Shen, Kaina1; Yuan, Dunxuan1; Fan, Jinping1; Yang, Yan1; Tian, Fangzhou1; Quan, Jinrou1; Li, Chengyun1; Wang, Junling1,2
2023-12
Online publication date2023-10
Source PublicationREPRODUCTIVE TOXICOLOGY   Impact Factor & Quartile
ISSN0890-6238 ; 1873-1708
EISSN1873-1708
Volume122
page numbers9
AbstractHistone modifications play important roles in the epigenetic regulation of spermatogenesis via mediating gene transcription. Steroidogenic regulatory enzymes control testosterone biosynthesis, which are essential for spermatogenesis. Arsenic exposure inhibits the expression of steroidogenic genes by significantly increasing trimethylation of H3K9 (H3K9me3) level in rat testis, finally diminishes testosterone release and lowers the rat sperm quality. Acetylation of H3K14 (H3K14ac) is associated with testosterone production and spermatogenesis. Co-occurrence of H3K9me3/H3K14ac has been identified previously by mass spectrometry in histone H3 isolated from different human cell types. H3K9me3/H3K14ac dually marked regions are in a poised inactive state to inhibit the gene expression. Whereas, whether inorganic arsenic exposure affects spermatogenesis and steroidogenic regulatory enzymes via mediating H3K14ac level has not been studied. Thereupon, the male SpragueDawley (SD) rats were exposed to (NaAsO2) for 6 weeks, then the sperm density and motility, testosterone level in serum, arsenic in rat testis were detected. mRNA expression of steroidogenic regulatory enzymes Star, Cyp11a1, Hsd3b and Hsd17b were determined by RT-PCR. H3K14ac level and the expression of histone acetylases of H3K14 (KAT2A and EP300), histone deacetylases of H3K14 (HDAC6 and HDAC3), the reader of H3K14ac (BAZ2A) were determined. The results suggested arsenic enhances H3K14ac in rat testis, which was associated with repression of steroidogenic regulatory genes expression, further reduced testosterone production, and impaired the spermatogenesis.
KeywordArsenic Steroidogenic regulatory enzymes Testosterone production Spermatogenesis
PublisherPERGAMON-ELSEVIER SCIENCE LTD
DOI10.1016/j.reprotox.2023.108474
Indexed BySCIE
Language英语
WOS Research AreaReproductive Biology ; Toxicology
WOS SubjectReproductive Biology ; Toxicology
WOS IDWOS:001087377600001
Original Document TypeArticle
PMID 37757915
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/569509
Collection兰州大学
Corresponding AuthorWang, Junling
Affiliation
1.Lanzhou Univ, Sch Publ Hlth, Lanzhou 730030, Peoples R China;
2.Lanzhou Univ Publ Hlth, Lab Toxicol, Lanzhou, Peoples R China
Recommended Citation
GB/T 7714
Li, Xiangli,Shen, Kaina,Yuan, Dunxuan,et al. Sodium arsenite exposure enhances H3K14 acetylation and impairs male spermatogenesis in rat testes[J]. REPRODUCTIVE TOXICOLOGY,2023,122.
APA Li, Xiangli.,Shen, Kaina.,Yuan, Dunxuan.,Fan, Jinping.,Yang, Yan.,...&Wang, Junling.(2023).Sodium arsenite exposure enhances H3K14 acetylation and impairs male spermatogenesis in rat testes.REPRODUCTIVE TOXICOLOGY,122.
MLA Li, Xiangli,et al."Sodium arsenite exposure enhances H3K14 acetylation and impairs male spermatogenesis in rat testes".REPRODUCTIVE TOXICOLOGY 122(2023).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[Li, Xiangli]'s Articles
[Shen, Kaina]'s Articles
[Yuan, Dunxuan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Xiangli]'s Articles
[Shen, Kaina]'s Articles
[Yuan, Dunxuan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Xiangli]'s Articles
[Shen, Kaina]'s Articles
[Yuan, Dunxuan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.