兰州大学机构库
Neuroprotective Effects of Vinpocetine Against Ischemia-Reperfusion Injury Via Inhibiting NLRP3 Inflammasome Signaling Pathway
Hou, Boru1; Li, Donghai3; Wang, Dengfeng1; Jiang, Cheng1; Wang, Gang1; Wang, Dong1; Yan, Guizhong1; Guo, Xiumei1; Zhao, Lixia1; Wan, Zhuangzhuang2; Fan, Chenlong2; Cao, Wencheng2; Ren, Haijun1
2023-08-21
Online publication date2023-07
Source PublicationNEUROSCIENCE   Impact Factor & Quartile
ISSN0306-4522 ; 1873-7544
Volume526Pages:74-84
page numbers11
AbstractIschemic stroke is one of the main causes of serious disability and death worldwide. NLRP3 inflamma-some is an intracellular pattern recognition receptor composed of polyprotein complex, which participates in mediating a series of inflammatory responses and is considered as a potential target for the treatment of ischemic stroke. Vinpocetine, a derivative of vincamine, has been widely used in the prevention and treatment of ischemic stroke. However, the therapeutic mechanism of vinpocetine is not clear, and its effect on NLRP3 inflammasome remains to be determined. In this study, we used the mouse model of transient middle cerebral artery occlusion (tMCAO) to simulate the occurrence of ischemic stroke. Different doses of vinpocetine (5, 10, 15 mg/kg/d) were injected intraperitoneally for 3 days after ischemia-reperfusion in mice. The effects of different doses of vin-pocetine on the degree of ischemia-reperfusion injury in mice were observed by TTC staining and modified neu-rological severity score scale, and the optimal dose was determined. Then, based on this optimal dose, we observed the effects of vinpocetine on apoptosis, microglial proliferation and NLRP3 inflammasome. In addition, we compared the effects of vinpocetine and MCC950 (a specific inhibitor of NLRP3 inflammasome) on NLRP3 inflammasome. Our results show that vinpocetine can effectively reduce the infarct volume and promote the recovery of behavioral function in stroke mice, and the maximal beneficial effects were observed at the dose of 10 mg/kg/d. Vinpocetine can effectively inhibit the apoptosis of peri-infarct neurons, promote the expression of Bcl-2, inhibit the expression of Bax and Cleaved Caspase-3, and reduce the proliferation of peri-infarct microglia. In addition, vinpocetine, like MCC950, can reduce the expression of NLRP3 inflammasome. Therefore, vinpocetine can effectively alleviate the ischemia-reperfusion injury in mice, and the inhibition of NLRP3 inflammasome may be an important therapeutic mechanism of vinpocetine.
Keywordvinpocetine NLRP3 inflammasome ischemic stroke ischemia-reperfusion
PublisherPERGAMON-ELSEVIER SCIENCE LTD
DOI10.1016/j.neuroscience.2023.05.021
Indexed BySCIE
Language英语
WOS Research AreaNeurosciences & Neurology
WOS SubjectNeurosciences
WOS IDWOS:001036541400001
Original Document TypeArticle
PMID 37290685
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/569643
Collection兰州大学
Corresponding AuthorHou, Boru; Ren, Haijun
Affiliation
1.Lanzhou Univ, Hosp 2, Dept Neurosurg, Lanzhou, Peoples R China;
2.Lanzhou Univ, Clin Med Coll 2, Lanzhou, Peoples R China;
3.Lanzhou Univ, Sch Life Sci, Gansu Key Lab Biomonitoring & Bioremediat Environm, Lanzhou, Peoples R China
Recommended Citation
GB/T 7714
Hou, Boru,Li, Donghai,Wang, Dengfeng,et al. Neuroprotective Effects of Vinpocetine Against Ischemia-Reperfusion Injury Via Inhibiting NLRP3 Inflammasome Signaling Pathway[J]. NEUROSCIENCE,2023,526:74-84.
APA Hou, Boru.,Li, Donghai.,Wang, Dengfeng.,Jiang, Cheng.,Wang, Gang.,...&Ren, Haijun.(2023).Neuroprotective Effects of Vinpocetine Against Ischemia-Reperfusion Injury Via Inhibiting NLRP3 Inflammasome Signaling Pathway.NEUROSCIENCE,526,74-84.
MLA Hou, Boru,et al."Neuroprotective Effects of Vinpocetine Against Ischemia-Reperfusion Injury Via Inhibiting NLRP3 Inflammasome Signaling Pathway".NEUROSCIENCE 526(2023):74-84.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[Hou, Boru]'s Articles
[Li, Donghai]'s Articles
[Wang, Dengfeng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Hou, Boru]'s Articles
[Li, Donghai]'s Articles
[Wang, Dengfeng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Hou, Boru]'s Articles
[Li, Donghai]'s Articles
[Wang, Dengfeng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.