| Ultrasound molecular imaging for early detection of acute renal ischemia–reperfusion injury | |
Ren, Ling1,2,3; Zhao, Yuzhuo2; Wang, Tiantian3; Tong, Yan3; Zhao, Ping2; Nie, Fang1; Luo, Yukun1,2 ; Zhu, Lianhua2 | |
| 2024 | |
| Online publication date | 2024-01 |
| Source Publication | Bioengineering and Translational Medicine Impact Factor & Quartile Of Published Year The Latest Impact Factor & Quartile |
| EISSN | 2380-6761 |
| page numbers | 13 |
| Abstract | Background: Microcirculatory perfusion disorder and inflammatory response are critical links in acute kidney injury (AKI). We aim to construct anti-vascular cell adhesion molecule-1(VCAM-1) targeted microbubbles (TM) to monitor renal microcirculatory perfusion and inflammatory response. Methods: TM carrying VCAM-1 polypeptide was constructed by biological coupling. The binding ability of TM to human umbilical vein endothelial cells (HUVECs) was detected. Bilateral renal ischemia–reperfusion injury (IRI) models of mice were established to evaluate microcirculatory perfusion and inflammatory response using TM. Thirty-six mice were randomly divided into six groups according to the different reperfusion time (0.5, 2, 6, 12, and 24 h) and sham-operated group (Sham group). The correlation of TM imaging with serum and histopathological biomarkers was investigated. Results: TM has advantages such as uniform distribution, regular shape, high stability, and good biosafety. TM could bind specifically to VCAM-1 molecule expressed by tumor necrosis factor-alpha (TNF-α)-treated HUVECs. In the renal IRI-AKI model, the area under the curve (AUC) of TM significantly decreased both in the renal cortical and medullary after 2 h of reperfusion compared with the Sham group (p © 2024 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. |
| Keyword | Cell adhesion Cell death Creatinine Diagnosis Endothelial cells Mammals Molecular imaging Molecules Ultrasonic applications Urea Acute kidney injury Human umbilical vein endothelial cells Inflammation Inflammatory response Injury models Ischemia-reperfusion injury Reperfusion Targeted microbubbles Ultrasound molecular imaging Vascular cell adhesion molecule-1 |
| Publisher | John Wiley and Sons Inc |
| DOI | 10.1002/btm2.10638 |
| Indexed By | EI ; SCIE |
| Language | 英语 |
| WOS Research Area | Biotechnology & Applied Microbiology ; Engineering ; Pharmacology & Pharmacy |
| WOS Subject | Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Pharmacology & Pharmacy |
| WOS ID | WOS:001138030900001 |
| EI Accession Number | 20240115327996 |
| EI Keywords | Biomarkers |
| EI Classification Number | 461.1 Biomedical Engineering ; 461.2 Biological Materials and Tissue Engineering ; 461.6 Medicine and Pharmacology ; 461.9 Biology ; 746 Imaging Techniques ; 753.3 Ultrasonic Applications ; 804.1 Organic Compounds ; 931.3 Atomic and Molecular Physics |
| Original Document Type | Article in Press |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.lzu.edu.cn/handle/262010/582825 |
| Collection | 兰州大学 |
| Corresponding Author | Luo, Yukun; Zhu, Lianhua |
| Affiliation | 1.The Second Medical College of Lanzhou University, Gansu, Lanzhou, China; 2.Department of Ultrasound, First Medical Center of Chinese PLA General Hospital, Beijing, China; 3.Department of Nephrology, First Medical Center of Chinese PLA General Hospital, Nephrology Institute of the Chinese People's Liberation Army, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Disease Research, Beijing, China |
| First Author Affilication | Lanzhou University |
| Corresponding Author Affilication | Lanzhou University |
| First Signature Affilication | Lanzhou University |
| Recommended Citation GB/T 7714 | Ren, Ling,Zhao, Yuzhuo,Wang, Tiantian,et al. Ultrasound molecular imaging for early detection of acute renal ischemia–reperfusion injury[J]. Bioengineering and Translational Medicine,2024. |
| APA | Ren, Ling.,Zhao, Yuzhuo.,Wang, Tiantian.,Tong, Yan.,Zhao, Ping.,...&Zhu, Lianhua.(2024).Ultrasound molecular imaging for early detection of acute renal ischemia–reperfusion injury.Bioengineering and Translational Medicine. |
| MLA | Ren, Ling,et al."Ultrasound molecular imaging for early detection of acute renal ischemia–reperfusion injury".Bioengineering and Translational Medicine (2024). |
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