兰州大学机构库 >化学化工学院
Selective fluorescent probes for live-cell monitoring of sulphide
Qian, Y; Karpus, J; Kabil, O; Zhang, SY; Zhu, HL; Banerjee, R; Zhao, J; He, C; He, C (reprint author), Univ Chicago, Dept Chem, 929 E 57th St, Chicago, IL 60637 USA.
ESI NOTE2017-07-19;Highly Cited Papers
2011-10
Source PublicationNATURE COMMUNICATIONS
ISSN2041-1723
Volume2
AbstractAqueous sulphides, including hydrogen sulphide, have important roles in biological signalling and metabolic processes. Here we develop a selective sulphide-trapping strategy involving sulphide addition to an aldehyde; the resulting hemithioacetal undergoes a Michael addition with an adjacent unsaturated acrylate ester to form a thioacetal at neutral pH in aqueous solution. Employing this new strategy, two sulphide-selective fluorescent probes, SFP-1 and SFP-2, were synthesized on the basis of two different fluorophore templates. These probes exhibit an excellent fluorescence increase and an emission maximum shift (SFP-1) in response to Na(2)S and H(2)S in a high thiol background as found under physiological conditions. We show the utility of the probes for the selective detection of sulphides, and the capacity of our probes to monitor enzymatic H(2)S biogenesis and image free sulphide in living cells.
Subject AreaScience & Technology - Other Topics
PublisherNATURE PUBLISHING GROUP
DOI10.1038/ncomms1506
Publication PlaceLONDON
Indexed BySCIE ; PubMed ; MEDLINE ; ESI
Language英语
First Inst
Funding Project国家自然科学基金项目 ; 国家留学基金委项目 ; 美国国家科学基金项目 ; 美国国立卫生研究院项目
Project NumberUS National Science Foundation International Collaboration in Chemistry between US Investigators and their Counterparts Abroad [CHE-0922998/NSFC 20921120404] ; National Institutes of Health [HL58984] ; China Scholar Program ; Chemistry-Biology Interface Predoctoral Training Grant ; Natural Science Foundation of China (NSFC) [20921120404]
WOS IDWOS:000296787300008
Funding OrganizationUS National Science Foundation International Collaboration in Chemistry between US Investigators and their Counterparts Abroad [CHE-0922998/NSFC 20921120404]; National Institutes of Health [HL58984]; China Scholar Program; Chemistry-Biology Interface Predoctoral Training Grant; Natural Science Foundation of China (NSFC) [20921120404] ; NSFC ; CSC ; US NSF ; NIH
SubtypeArticle
PMID 21988911
Department
[Qian, Yong;
Karpus, Jason;
Zhang, Shu-Yu;
He, Chuan] Univ Chicago, Dept Chem, Chicago, IL 60637 USA;
[Qian, Yong;
Karpus, Jason;
Zhang, Shu-Yu;
He, Chuan] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA;
[Qian, Yong;
Zhu, Hai-Liang...更多
Citation statistics
Cited Times:428[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/75993
Collection化学化工学院
Corresponding AuthorHe, C (reprint author), Univ Chicago, Dept Chem, 929 E 57th St, Chicago, IL 60637 USA.
Recommended Citation
GB/T 7714
Qian, Y,Karpus, J,Kabil, O,et al. Selective fluorescent probes for live-cell monitoring of sulphide[J]. NATURE COMMUNICATIONS,2011,2.
APA Qian, Y.,Karpus, J.,Kabil, O.,Zhang, SY.,Zhu, HL.,...&He, C .(2011).Selective fluorescent probes for live-cell monitoring of sulphide.NATURE COMMUNICATIONS,2.
MLA Qian, Y,et al."Selective fluorescent probes for live-cell monitoring of sulphide".NATURE COMMUNICATIONS 2(2011).
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