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Interactions between 1-benzoyl-4-p-chlorophenyl thiosemicarbazide and serum albumin: investigation by fluorescence spectroscopy
Cui, FL; Fan, J; Li, JP; Hu, ZD(胡之德); Cui, FL (reprint author), Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China.
2004-01-02
Source PublicationBIOORGANIC & MEDICINAL CHEMISTRY
ISSN0968-0896
Volume12Issue:1
AbstractThe interactions between 1-benzoyl-4-p-chlorphenyl thiosemicarbazide (BCPT) and bovine serum albumin (BSA) or human serum albumin (HSA) have been studied by fluorescence spectroscopy. By the analysis of fluorescence spectrum and fluorescence intensity, it was showed that BCPT has a strong ability to quench the intrinsic fluorescence of both bovine serum albumin and human serum albumin through a static quenching procedure. The binding constants of BCPT with BSA or HSA were determined at different temperatures based on the fluorescence quenching results. The binding sites were obtained and the binding force were suggested to be mainly hydrophobic. The effect of common ions on the binding constants was also investigated. A new fluorescence spectroscopy assay of the proteins is presented. The linear range is 5.36-67.0 mug mL(-1) with recovery of 101.1% for BSA, and the linear range is 8.28-144.9 mug mL(-1) with recovery of 102.6% for HSA. Determination of the proteins in bovine serum or in human serum by this method gives results which are very close to those obtained by using Coomassie Brilliant Blue G-250 colorimetry. A practical method was proposed for the determination of BCPT in human serum samples. (C) 2003 Elsevier Ltd. All rights reserved.
Keyword1-benzoyl-4-p-chlorphenyl thiosemicarbazide (BCPT) bovine serum albumin (BSA) human serum albumin (HSA) fluorescence spectroscopy
Subject AreaBiochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Chemistry
PublisherELSEVIER
DOI10.1016/j.bme.2003.10.018
Publication PlaceOXFORD
Indexed BySCIE ; PubMed ; MEDLINE ; BIOSIS
Language英语
First Inst
WOS IDWOS:000188113400016
SubtypeArticle
PMID 14697780
BIOSIS IDBIOSIS:PREV200400165028
DepartmentLanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China;
Henan Normal Univ, Sch Chem & Environm Sci, Xinxiang 453002, Henan, Peoples R China
Citation statistics
Cited Times:335[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/79081
Collection化学化工学院
Corresponding AuthorCui, FL (reprint author), Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China.
Recommended Citation
GB/T 7714
Cui, FL,Fan, J,Li, JP,et al. Interactions between 1-benzoyl-4-p-chlorophenyl thiosemicarbazide and serum albumin: investigation by fluorescence spectroscopy[J]. BIOORGANIC & MEDICINAL CHEMISTRY,2004,12(1).
APA Cui, FL,Fan, J,Li, JP,Hu, ZD,&Cui, FL .(2004).Interactions between 1-benzoyl-4-p-chlorophenyl thiosemicarbazide and serum albumin: investigation by fluorescence spectroscopy.BIOORGANIC & MEDICINAL CHEMISTRY,12(1).
MLA Cui, FL,et al."Interactions between 1-benzoyl-4-p-chlorophenyl thiosemicarbazide and serum albumin: investigation by fluorescence spectroscopy".BIOORGANIC & MEDICINAL CHEMISTRY 12.1(2004).
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